Details, Fiction and thr77
Details, Fiction and thr77
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which ends up in chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in promoting mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two further splice variants were described but comprehensive nucleotide sequence information and facts just isn't nevertheless offered.
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Blocking peptides are peptides that bind precisely for the target antibody and block antibody binding. These peptide usually includes the epitope recognized by the antibody.
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This information outlines even more possible optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the get more info staining through the blocked antibody versus the antibody by yourself, you can see which staining is distinct.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin relatives, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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